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StaphyType 96

DNA Array Hybridisation Kit for S.aureus Resistance Gene & Pathogenicity Marker Detection

StaphyType96 Kit is designed to help

  • epidemiologists, who need information about the phylogenetic relationship of S.aureus strains in distinct geographic regions, about the dissemination of individual strains, or about the loss or gain of distinct genetic markers in S. aureus strains,
  • hygienists, who want to learn, whether infections are caused by one identical or by different S.aureus strains, and who rapidly need to identify an evolving strain to prevent outbreaks,
  • veterinary institutions, forensic medicine, food industry to survey and control for potential contaminations.

Background

Staphylococcus aureus is a major cause of hospital-acquired (nosocomial) infections with an increasing evolution of antibiotic resistance and potentially life-threatening virulence factors. Nosocomial infection is one of the major causes of death and serious illness worldwide. In addition, community acquired MRSA (caMRSA) strains have evolved transferring the problem of antibiotic resistance in S. aureus strains from hospital patients to other groups like nursing home residents, athletic teams, military recruits, and inmates of correctional facilities. S.aureus can cause severe adversity in animal flocks, and its toxins are relevant in food industry.

These problems have resulted in an increased demand for tests enabling the rapid and precise identification of evolving S.aureus germs.

Assay Principle

The StaphyType 96 Kit allows the reliable detection of the most common resistance genes, pathogenicity and strain-specific markers of S.aureus strains, thus allowing the precise strain assignment of unknown isolates.

Central unit of the test are ArrayStrips provided with a high quality micro array integrated at the bottom of each well. Each StaphyType array carries a set of 333 different probes representing S.aureus resistance genes and other markers. Biotin-labeled clonal S.aureus DNA isolated from overnight cultures is hybridised against the micro arrays resulting in strain specific hybridisation patterns. Detection of the pattern is performed applying the method of precipitation staining. For image recording and automated analysis, the ArrayMate device is available. With its specific analysis software, final test reports including precise strain assignment are automatically generated.

Starting with bacterial overnight culture the test is performed within one working day in a hands-on time of approximately 50 to 80 minutes.

StaphyType 96 Kit Content

  • 12 ArrayStrips S.aureus (for 96 tests)
  • Lysis Reagent
  • Labeling Reagents
  • Hybridisation Buffer
  • Washing Buffers
  • HRP Conjugate Reagents
  • HRP Detection Solution
  • Positive Controls

For order information please contact us.